Journal: Cerebral cortex (New York, N.Y. : 1991)

Article Title: The DNA Methyltransferase 1 (DNMT1) Controls the Shape and Dynamics of Migrating POA-Derived Interneurons Fated for the Murine Cerebral Cortex.

doi: 10.1093/cercor/bhw341

Figure Lengend Snippet: Figure 5 Postmitotic Dnmt1 deletion in POA-derived interneurons leads to migratory defects during embryonic development. (A, B) Location and number of Hmx3-Cre/

Article Snippet: For siRNA transfection of N2a cells, reverse lipofection with Lipofectamin 2000 (Thermo Fisher Scientific) was performed with 50 nM control siRNA (BLOCK-iT Alexa Fluor red fluorescent oligo, 465318; Invitrogen), Dnmt1 or Pak6 siRNA (Pak6 siRNA sc44879; Dnmt1 siRNA sc-35203; Santa Cruz Biotechnology) for 5 h in Opti-MEM I Reduced Serum Medium (Thermo Fisher Scientific).

Techniques: Derivative Assay

Journal: Cerebral cortex (New York, N.Y. : 1991)

Article Title: The DNA Methyltransferase 1 (DNMT1) Controls the Shape and Dynamics of Migrating POA-Derived Interneurons Fated for the Murine Cerebral Cortex.

doi: 10.1093/cercor/bhw341

Figure Lengend Snippet: Figure 6 Dnmt1 deficiency causes morphological abnormalities and impairments of the migratory capacity. (A, B) Inverted microphotographs and drawings of repre-

Article Snippet: For siRNA transfection of N2a cells, reverse lipofection with Lipofectamin 2000 (Thermo Fisher Scientific) was performed with 50 nM control siRNA (BLOCK-iT Alexa Fluor red fluorescent oligo, 465318; Invitrogen), Dnmt1 or Pak6 siRNA (Pak6 siRNA sc44879; Dnmt1 siRNA sc-35203; Santa Cruz Biotechnology) for 5 h in Opti-MEM I Reduced Serum Medium (Thermo Fisher Scientific).

Techniques:

Journal: Cerebral cortex (New York, N.Y. : 1991)

Article Title: The DNA Methyltransferase 1 (DNMT1) Controls the Shape and Dynamics of Migrating POA-Derived Interneurons Fated for the Murine Cerebral Cortex.

doi: 10.1093/cercor/bhw341

Figure Lengend Snippet: Figure 7 Noncanonical actions of DNMT1 regulate Pak6 expression level affecting the morphology of postmitotic POA cells. (A) Schematic illustration of the region

Article Snippet: For siRNA transfection of N2a cells, reverse lipofection with Lipofectamin 2000 (Thermo Fisher Scientific) was performed with 50 nM control siRNA (BLOCK-iT Alexa Fluor red fluorescent oligo, 465318; Invitrogen), Dnmt1 or Pak6 siRNA (Pak6 siRNA sc44879; Dnmt1 siRNA sc-35203; Santa Cruz Biotechnology) for 5 h in Opti-MEM I Reduced Serum Medium (Thermo Fisher Scientific).

Techniques: Expressing

Journal: BMC Cancer

Article Title: The role of DNMT1/hsa-miR-124-3p/BCAT1 pathway in regulating growth and invasion of esophageal squamous cell carcinoma

doi: 10.1186/s12885-019-5815-x

Figure Lengend Snippet: The regulatory role of DNMT1 inhibitor on expression of hsa-miR-124-3p and BCAT1 in ESCC cells. a - b The mRNA expression of DNMT1 in normal tissues and ESCC tissues ( a ) and its expression in HEEC, KYSE-150 and Eca109 cells ( b ) were determined by qRT-PCR analysis. c The methylation status of hsa-miR-124-3p in KYSE-150 and Eca109 cells treated with DNMT1 inhibitor 5 μM 5-Azacitidine (5-Aza) or its control (PBS), was detected by methylation specific PCR. d The mRNA expression of DNMT1, hsa-miR-124-3p and BCAT1 in KYSE-150 and Eca109 cells treated with 5-Aza or PBS were determined by qRT-PCR analysis. e The protein expression of DNMT1 and BCAT1 were detected by western blotting assay. The target protein expression relative to GAPDH expression was displayed on the right. The experiments were performed in triplicate and each value represents mean ± SD. *** P < 0.001

Article Snippet: For cell transfection, siRNAs including DNMT1 siRNA, BCAT1 siRNA, scrambled siRNA (NC siRNA), miR-124-3p mimics and its negative control (NC mimics) were supplied by Ribobio Company (Guangzhou, China) and pcDNA-BCAT1(OE-BCAT1) or pcDNA-negative (OE-NC) were constructed using pcDNA3.1(+) vector.

Techniques: Expressing, Quantitative RT-PCR, Methylation, Control, Western Blot

Journal: BMC Cancer

Article Title: The role of DNMT1/hsa-miR-124-3p/BCAT1 pathway in regulating growth and invasion of esophageal squamous cell carcinoma

doi: 10.1186/s12885-019-5815-x

Figure Lengend Snippet: The regulatory role of DNMT1 siRNA on hsa-miR-124-3p / BCAT1 in ESCC cells. a The methylation status of hsa-miR-124-3p in KYSE-150 and Eca109 cells transfected with DNMT1 siRNA or NC siRNA was detected by methylation specific PCR. b The mRNA expression of DNMT1, hsa-miR-124-3p and BCAT1 in KYSE-150 and Eca109 cells transfected with DNMT1 siRNA or NC siRNA were determined by qRT-PCR analysis. c - d The protein expression of DNMT1 and BCAT1 were detected by western blotting assay ( c ) and immunofluorescence staining assay ( d ) respectively. The target protein expression relative to GAPDH expression was displayed on the right of Fig. 8c. The experiments were performed in triplicate and each value represents mean ± SD. *** P < 0.001

Article Snippet: For cell transfection, siRNAs including DNMT1 siRNA, BCAT1 siRNA, scrambled siRNA (NC siRNA), miR-124-3p mimics and its negative control (NC mimics) were supplied by Ribobio Company (Guangzhou, China) and pcDNA-BCAT1(OE-BCAT1) or pcDNA-negative (OE-NC) were constructed using pcDNA3.1(+) vector.

Techniques: Methylation, Transfection, Expressing, Quantitative RT-PCR, Western Blot, Immunofluorescence, Staining